Circulating CD14 + CD163 + CD206 + M2 Monocytes Are Increased in Patients with Early Stage of Idiopathic Membranous Nephropathy

Mediators Inflamm. 2018 Jun 21;2018:5270657. doi: 10.1155/2018/5270657. eCollection 2018.

Abstract

Aim: To analyze changes in peripheral blood monocytes and their clinical significance in patients with early stage of idiopathic membranous nephropathy (IMN).

Methods: A total of 27 patients with early stage of IMN and 16 age- and sex-matched healthy controls (HCs) were recruited for the study. The monocyte subset counts in circulation were measured by flow cytometry, and serum interleukin- (IL-) 10 and IL-12 concentrations were tested by enzyme-linked immunosorbent assay. The potential association between clinical signs and monocyte subset counts was analyzed statistically.

Results: Compared with the HCs, the patients with early stage of IMN had higher counts of CD14+CD163+, CD14+CD163+CD206+, and CD14+CD163+CD206+CD115+ M2-like monocytes. The CD14+CD163+CD206+ M2-like cell counts and intracellular IL-10 concentrations in the monocytes were positively correlated with progression in proteinuria. The levels of serum IL-10 were significantly higher in early IMN patients than in the HCs. Furthermore, CD14+CD163+CD206+ M2-like cell counts in the patients with incipient IMN were also positively related with 24 h urinary albumin levels and the values of serum M-type phospholipase A2 receptor (PLA2R).

Conclusion: CD14+CD163+CD206+ M2-like monocytes may contribute to the pathologic process in early-stage IMN and could serve as potential markers for evaluating the disease severity.

MeSH terms

  • Adult
  • Antigens, CD / metabolism
  • Antigens, Differentiation, Myelomonocytic / metabolism
  • Case-Control Studies
  • Enzyme-Linked Immunosorbent Assay
  • Female
  • Flow Cytometry
  • Glomerulonephritis, Membranous / immunology*
  • Humans
  • Interleukin-10 / metabolism
  • Lectins, C-Type / metabolism
  • Leukocyte Count
  • Lipopolysaccharide Receptors / metabolism
  • Male
  • Mannose Receptor
  • Mannose-Binding Lectins / metabolism
  • Middle Aged
  • Monocytes / cytology*
  • Monocytes / metabolism
  • Receptors, Cell Surface / metabolism
  • Receptors, Phospholipase A2 / metabolism

Substances

  • Antigens, CD
  • Antigens, Differentiation, Myelomonocytic
  • CD163 antigen
  • IL10 protein, human
  • Lectins, C-Type
  • Lipopolysaccharide Receptors
  • Mannose Receptor
  • Mannose-Binding Lectins
  • PLA2R1 protein, human
  • Receptors, Cell Surface
  • Receptors, Phospholipase A2
  • Interleukin-10