Engineering Lactobacillus rhamnosus GG and GR-1 to express HIV-inhibiting griffithsin

Int J Antimicrob Agents. 2018 Nov;52(5):599-607. doi: 10.1016/j.ijantimicag.2018.07.013. Epub 2018 Jul 21.

Abstract

Probiotic bacteria are being explored for the in situ delivery of various therapeutic agents. In this study, we aimed to express two HIV-inhibiting lectins, actinohivin (AH) and griffithsin (GRFT), in the probiotic strains Lactobacillus rhamnosus GG and L. rhamnosus GR-1 for gastrointestinal and vaginal mucosal delivery, respectively. Constructs were generated for the intracellular and extracellular production of AH and GRFT under the control of the promoter of their Major Secreted Protein Msp1. Also, intracellular expression of GRFT was investigated under the control of the nisA promoter from the inducible nisin-controlled expression (NICE) system. For the extracellular localization, the signal leader peptide of Msp1/p75 from L. rhamnosus GG was translationally fused with the genes encoding AH and GRFT. Construction of recombinant strains expressing the AH monomer and dimer was unsuccessful, probably due to the intracellular toxicity of AH for the lactobacilli. On the other hand, recombinant strains for intra- and extracellular production of GRFT by L. rhamnosus GG and GR-1 were successfully constructed. The highest expression levels of recombinant GRFT were observed for the constructs under the control of the inducible nisA promoter and we demonstrated anti-HIV activity against an M-tropic and a T-tropic HIV-1 strain. We can conclude that recombinant Lactobacillus expressing anti-HIV lectins could contribute to the development of enhanced probiotic strains that are able to inhibit HIV transmission and subsequent replication, although further research and development are required.

Keywords: Actinohivin; Carbohydrate-binding agents; Griffithsin; HIV; Lactobacillus rhamnosus; Probiotics.

MeSH terms

  • Anti-HIV Agents / metabolism*
  • Bacterial Proteins / genetics
  • Bacterial Proteins / metabolism
  • Cell Line
  • Gene Expression
  • HIV-1 / drug effects
  • HIV-1 / physiology
  • Humans
  • Lactobacillus rhamnosus / genetics
  • Lactobacillus rhamnosus / metabolism*
  • Metabolic Engineering / methods*
  • Plant Lectins / genetics
  • Plant Lectins / metabolism*
  • Promoter Regions, Genetic
  • Protein Sorting Signals
  • Recombinant Proteins / genetics
  • Recombinant Proteins / metabolism
  • Virus Replication / drug effects

Substances

  • Anti-HIV Agents
  • Bacterial Proteins
  • Plant Lectins
  • Protein Sorting Signals
  • Recombinant Proteins
  • actinohivin protein, actinomycete
  • griffithsin protein, Griffithsia