Functional Genomics and Seed Development in Medicago truncatula: An Overview

Methods Mol Biol. 2018:1822:175-195. doi: 10.1007/978-1-4939-8633-0_13.

Abstract

The study of seed development in the model species Medicago truncatula has made a significant contribution to our understanding of this process in crop legumes. Thanks to the availability of comprehensive proteomics and transcriptomics databases, coupled with exhaustive mutant collections, the roles of several regulatory genes in development and maturation are beginning to be deciphered and functionally validated. Advances in next-generation sequencing and the availability of a genomic sequence have made feasible high-density SNP genotyping, allowing the identification of markers tightly linked to traits of agronomic interest. A further major advance is to be expected from the integration of omics resources in functional network construction, which has been used recently to identify "hub" genes central to important traits.

Keywords: AFL; Desiccation tolerance; Dormancy; Flavonoid; GWAS; Globulin; Longevity; Network.

Publication types

  • Review

MeSH terms

  • Gene Expression Profiling
  • Gene Expression Regulation, Plant
  • Gene Regulatory Networks
  • Genome, Plant*
  • Genome-Wide Association Study
  • Genomics* / methods
  • High-Throughput Nucleotide Sequencing
  • Medicago truncatula / genetics*
  • Medicago truncatula / metabolism
  • Mutation
  • Plant Development / genetics*
  • Plant Physiological Phenomena
  • Proteomics
  • Reproducibility of Results
  • Seeds / genetics*
  • Transcriptome