Isolation and characterization of a novel cytochrome P-450-like pseudogene

Biochem Biophys Res Commun. 1986 Jan 29;134(2):499-505. doi: 10.1016/s0006-291x(86)80448-x.

Abstract

A rabbit liver P-450-like pseudogene has been isolated from a lambda phage genomic library. Sequence analysis revealed structural homology with respect to the rat P-450b and P-450e genes as well as a similar intron-exon organization. A 5'-proximal TATA box-like sequence and two 3'-distal putative polyadenylation signals were identified, and all putative intron-exon boundaries except at the 3'-splice site of intron 2 were found to follow the GT/AG rule. With allowance for apparent deletions and insertions, the structural homology of the amino acid sequence deduced from the pseudogene with respect to rabbit P-450 isozyme 2 is lower for exons 1 through 4 (18-28%) than for exons 5 through 9 (42-65%). S1 nuclease mapping showed that mRNAs complementary to the DNA sequence of exon 9 are expressed. However, due to the alterations in the pseudogene, it appears that functional P-450 would not be produced from such mRNAs.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Chromosome Mapping
  • Cloning, Molecular
  • Cytochrome P-450 Enzyme System / genetics*
  • DNA / genetics
  • DNA Restriction Enzymes
  • Endonucleases
  • Phenobarbital
  • Rabbits
  • Sequence Homology, Nucleic Acid
  • Single-Strand Specific DNA and RNA Endonucleases

Substances

  • DNA
  • Cytochrome P-450 Enzyme System
  • Endonucleases
  • DNA Restriction Enzymes
  • Single-Strand Specific DNA and RNA Endonucleases
  • Phenobarbital

Associated data

  • GENBANK/M12280
  • GENBANK/M12281
  • GENBANK/M12282
  • GENBANK/M12283
  • GENBANK/M12284
  • GENBANK/M12285
  • GENBANK/M12286
  • GENBANK/M12287
  • GENBANK/M12288