Infant Mouse Model of Vibrio cholerae Infection and Colonization

Methods Mol Biol. 2018:1839:147-152. doi: 10.1007/978-1-4939-8685-9_13.


Cholera is an epidemic diarrheal disease caused by Vibrio cholerae that continues to cause significant morbidity and mortality in many parts of the world. Several different animal models have been used by scientists over the years to study the pathogenesis of cholera. However, the most commonly used is the infant (suckling) mouse model, which has been found to replicate important aspects of human intestinal colonization. Here we present a detailed protocol for using the infant mouse model to assess the colonization of V. cholerae strains using a competition assay.

Keywords: Colonization; Competitive index; Mouse model; Vibrio cholerae.

MeSH terms

  • Animals
  • Bacterial Load*
  • Cholera / microbiology*
  • Disease Models, Animal
  • Intestines / microbiology
  • Mice
  • Vibrio cholerae / physiology*