Different localization of the product of the v-rel oncogene in chicken fibroblasts and spleen cells correlates with transformation by REV-T

Cell. 1986 Mar 14;44(5):791-800. doi: 10.1016/0092-8674(86)90845-7.

Abstract

Reticuloendotheliosis virus strain T (REV-T) is a highly oncogenic avian retrovirus that transforms early lymphoid cells in vivo and in vitro, but REV-T does not transform chicken embryo fibroblasts (CEF). Using antisera to p59v-rel, the v-rel oncogene product of REV-T, we show that p59v-rel is expressed at equal levels and is a phosphoprotein in REV-T infected spleen cells and CEF. Biochemical fractionation and immunofluorescence of REV-T infected nontransformed CEF show that p59v-rel is loosely associated with the nucleus. However, in REV-T transformed spleen cells p59v-rel is primarily a cytoplasmic protein. MSB-1 cells, a Marek's disease virus transformed T cell leukemic line, and E26 virus transformed myeloid cells show nuclear staining of p59v-rel when they are infected by REV-T. Our results indicate that there is a correlation between a cytoplasmic localization of p59v-rel and transformation by REV-T, and they suggest that p59v-rel cannot transform cells in which it assumes solely a nuclear location.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Cell Compartmentation
  • Cell Nucleus / metabolism
  • Cell Transformation, Viral*
  • Cytoplasm / metabolism
  • Fibroblasts / physiology
  • Fluorescent Antibody Technique
  • Oncogene Proteins, Viral / immunology
  • Oncogene Proteins, Viral / metabolism*
  • Oncogenes*
  • Phosphoproteins / genetics
  • Phosphoproteins / immunology
  • Phosphoproteins / metabolism
  • Reticuloendotheliosis virus / genetics*
  • Retroviridae / genetics*
  • Spleen / physiology

Substances

  • Oncogene Proteins, Viral
  • Phosphoproteins