Traumatic brain injury (TBI) contributes to morbidity in children, and boys are disproportionately represented. Cerebral blood flow (CBF) is reduced and autoregulation is impaired after TBI, contributing to poor outcome. Cerebral perfusion pressure (CPP) is often normalized by use of vasoactive agents to increase mean arterial pressure (MAP). In prior studies of male and female newborn and juvenile pigs, we observed that phenylephrine, norepinephrine, epinephrine, and dopamine demonstrated different sex- and age-dependent abilities to prevent impairment of cerebral autoregulation and limit histopathology after TBI, despite equivalent CPP values. This observation complicated treatment choice. Alternatively, administration of a cerebral vasodilator may improve cerebral hemodynamics after TBI by increasing CBF. In prior studies, intravenous sodium nitroprusside, a nitric oxide (NO) releaser, elevated CBF after TBI but failed to prevent impairment of cerebral autoregulation due to a confounding decrease in MAP, which lowered CPP. We presently test the hypothesis that inhaled NO (iNO) will protect cerebral autoregulation and prevent hippocampal histopathology after TBI. Results show that iNO administered at 30 min or 2 h after TBI protected cerebral autoregulation and prevented neuronal cell necrosis in CA1 and CA3 hippocampus equivalently in male and female newborn and juvenile pigs without change in MAP. Protection lasted for at least 2 h after iNO administration was stopped. Papaverine-induced dilation was unchanged by TBI and iNO. These data indicate that iNO offers the opportunity to have a single therapeutic that uniformly protects autoregulation and limits hippocampal neuronal cell necrosis across both ages and sexes.
Keywords: age; brain injury; cerebral autoregulation; histopathology; nitric oxide; sex.