Seipin and the membrane-shaping protein Pex30 cooperate in organelle budding from the endoplasmic reticulum

Nat Commun. 2018 Jul 27;9(1):2939. doi: 10.1038/s41467-018-05278-2.


Lipid droplets (LDs) and peroxisomes are ubiquitous organelles with central roles in eukaryotic cells. Although the mechanisms involved in biogenesis of these organelles remain elusive, both seem to require the endoplasmic reticulum (ER). Here we show that in yeast the ER budding of these structurally unrelated organelles has remarkably similar requirements and involves cooperation between Pex30 and the seipin complex. In the absence of these components, budding of both LDs and peroxisomes is inhibited, leading to the ER accumulation of their respective constituent molecules, such as triacylglycerols and peroxisomal membrane proteins, whereas COPII vesicle formation remains unaffected. This phenotype can be reversed by remodeling ER phospholipid composition highlighting a key function of these lipids in organelle biogenesis. We propose that seipin and Pex30 act in concert to organize membrane domains permissive for organelle budding, and that may have a lipid composition distinct from the bulk ER.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • COP-Coated Vesicles / metabolism
  • Cell Division / physiology*
  • Endoplasmic Reticulum / metabolism*
  • GTP-Binding Protein gamma Subunits / genetics
  • GTP-Binding Protein gamma Subunits / metabolism*
  • Intracellular Membranes / metabolism
  • Lipid Droplets / metabolism
  • Membrane Proteins / genetics
  • Membrane Proteins / metabolism*
  • Mitochondrial Proteins / genetics
  • Mitochondrial Proteins / metabolism
  • Mutation
  • Organelle Biogenesis*
  • Peroxisomes / metabolism
  • Phospholipids / metabolism
  • Reproduction, Asexual / physiology*
  • Saccharomyces cerevisiae / cytology
  • Saccharomyces cerevisiae / metabolism
  • Saccharomyces cerevisiae Proteins / genetics
  • Saccharomyces cerevisiae Proteins / metabolism*
  • Triglycerides / metabolism


  • Fld1 protein, S cerevisiae
  • GTP-Binding Protein gamma Subunits
  • LDB16 protein, S cerevisiae
  • Membrane Proteins
  • Mitochondrial Proteins
  • Pex30 protein, S cerevisiae
  • Phospholipids
  • Saccharomyces cerevisiae Proteins
  • Triglycerides
  • seipin protein, S cerevisiae