Effect of Dietary Content of Menhaden Oil with or without Salsalate on Neuropathic Endpoints in High-Fat-Fed/Low-Dose Streptozotocin-Treated Sprague Dawley Rats

J Diabetes Res. 2018 Jul 2;2018:2967127. doi: 10.1155/2018/2967127. eCollection 2018.


In this study, we wanted to extend our investigation of the efficacy of fish oil with or without salsalate on vascular and neural complications using a type 2 diabetic rat model. Four weeks after the onset of hyperglycemia, diabetic rats were treated via the diet with 3 different amounts of menhaden oil with or without salsalate for 12 weeks. Afterwards, vascular reactivity of epineurial arterioles and neuropathy-related endpoints were examined. The addition of salsalate to high-fat diets enriched with 10% or 25% kcal of menhaden oil protected vascular reactivity to acetylcholine and calcium gene-related peptide, motor and sensory nerve conduction velocity, thermal nociception, intraepidermal nerve fiber density, and cornea sensitivity to a greater extent than 10% or 25% menhaden oil alone. Vascular and neural function was maximally protected with diet containing 45% kcal as menhaden oil, and adding salsalate did not provide any additional benefit. Salsalate alone in the high-fat diet of diabetic rats provided minimal protection/improvement of vascular and neural dysfunction. These studies imply that dietary salsalate in combination with lower amounts of menhaden oil can provide greater benefit toward diabetes-induced vascular and neural impairment than menhaden oil alone.

MeSH terms

  • Animals
  • Blood Glucose
  • Diabetes Mellitus, Experimental / drug therapy*
  • Diabetic Neuropathies / drug therapy*
  • Diet*
  • Diet, Fat-Restricted
  • Diet, High-Fat
  • Disease Models, Animal
  • Fish Oils / pharmacology*
  • Male
  • Peripheral Nervous System Diseases
  • Rats
  • Rats, Sprague-Dawley
  • Salicylates / pharmacology*
  • Sciatic Nerve / pathology


  • Blood Glucose
  • Fish Oils
  • Salicylates
  • Menhaden oil
  • salicylsalicylic acid