Mass Spectrometric Imaging of GABA in the Drosophila melanogaster Adult Head

Anal Sci. 2018 Sep 10;34(9):1055-1059. doi: 10.2116/analsci.18SCN01. Epub 2018 Jul 27.

Abstract

Drosophila melanogaster is a model organism in neurodegenerative disease research. In neurodegenerative study, the direct spatial information of neurotransmitters such as γ-aminobutyric acid (GABA) in the brain of Drosophila melanogaster is important to understand the role of GABA. Matrix-assisted laser desorption/ionization imaging mass spectrometry (MALDI-IMS) is an attractive method for direct visualization of neurotransmitters. In this paper, we describe methods to visualize GABA in the brain and head of Drosophila melanogaster using MALDI-IMS.

Keywords: Drosophila melanogaster; GABA; Imaging mass spectrometry; on-tissue derivatization.

MeSH terms

  • Animals
  • Brain / diagnostic imaging
  • Brain / metabolism
  • Drosophila melanogaster / metabolism*
  • Molecular Imaging / methods*
  • Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization*
  • gamma-Aminobutyric Acid / metabolism*

Substances

  • gamma-Aminobutyric Acid