Nucleotide sequence of the hygromycin B phosphotransferase gene from Streptomyces hygroscopicus

Nucleic Acids Res. 1986 Feb 25;14(4):1565-81. doi: 10.1093/nar/14.4.1565.


The nucleotide sequence of a 1467 bp fragment of Streptomyces hygroscopicus DNA containing the gene (hyg) encoding a hygromycin B phosphotransferase (HPH) has been determined. The N-terminal amino acid sequence of HPH determined by automated Edman degradation has allowed the coding sequence of the hyg gene to be identified. The translation initiation triplet is GTG and 5 bp preceding it there is a sequence complementary to the 3'-end of 16S rRNA from S. lividans. The transcriptional start and termination sites have been determined; the presumptive promoter region has only partial homology to that of the Streptomyces vinaceus vph gene and is different to the promoter sequences of other Streptomyces genes.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • Chromosome Mapping
  • Codon
  • DNA, Bacterial / genetics
  • Genes
  • Genes, Bacterial
  • Genetic Vectors
  • Kanamycin Kinase
  • Nucleic Acid Conformation
  • Phosphotransferases / genetics*
  • Streptomyces / enzymology*
  • Streptomyces / genetics
  • Transcription, Genetic


  • Codon
  • DNA, Bacterial
  • Phosphotransferases
  • Kanamycin Kinase

Associated data

  • GENBANK/X03615