siRNA of DNA polymerase iota inhibits the migration and invasion in the lung cancer cell A549

Acta Biochim Biophys Sin (Shanghai). 2018 Sep 1;50(9):929-933. doi: 10.1093/abbs/gmy089.


DNA polymerase iota (polɩ) is a member of low-fidelity Y-family of DNA polymerases. Our previous studies have demonstrated that the overexpression of polι is associated with the poorer prognosis in lung cancer patients. Here, we designed the small interfering RNA (siRNA) targeting polɩ gene (POLI) to investigate the effect of polɩ on the proliferation, apoptosis, and invasion of the lung cancer cell line A549 in order to reveal the role of polι in lung cancer progression. Our results showed that siRNA of POLI had no significant effect on the proliferation and apoptosis of the lung cancer cell line A549. However, siRNA of POLI could inhibit the migration and invasion of the lung cancer cell line A549 by upregulating the E-cadherin expression and downregulating the expressions of N-cadherin, MMP2, and MMP9. Together, our findings indicate that polι plays a positive role in lung cancer progression via promoting the migration and invasion of lung cancer cells. Therefore, polι might be a potential target for the clinical treatment of lung cancer in the future.

MeSH terms

  • A549 Cells
  • Cadherins / metabolism
  • Cell Movement / genetics*
  • DNA Polymerase iota
  • DNA-Directed DNA Polymerase / genetics*
  • DNA-Directed DNA Polymerase / metabolism
  • Humans
  • Lung Neoplasms / enzymology
  • Lung Neoplasms / genetics
  • Lung Neoplasms / pathology
  • Matrix Metalloproteinase 2 / metabolism
  • Matrix Metalloproteinase 9 / metabolism
  • Neoplasm Invasiveness
  • RNA Interference*
  • RNA, Small Interfering / genetics*


  • Cadherins
  • RNA, Small Interfering
  • DNA-Directed DNA Polymerase
  • Matrix Metalloproteinase 2
  • Matrix Metalloproteinase 9
  • DNA Polymerase iota
  • POLI protein, human