Preliminary randomized controlled trial of antiaging effects of l-ascorbic acid applied in combination with no-needle and microneedle mesotherapy

J Cosmet Dermatol. 2019 Jun;18(3):843-849. doi: 10.1111/jocd.12727. Epub 2018 Aug 2.


Background: The epidermis is keratinized stratified squamous epithelium covered by hydro-lipid barrier. Vitamin C is a water-soluble antioxidant which protects skin from oxidative damage and rejuvenates photo-aged skin. There are different options of improving active substance penetration through the stratum corneum of the epidermis. One of them is noneedle mesotherapy which is a non-invasive rejuvenation technique involving electric pulses, electroporation, and ultrasounds. The use of these physicals factors results in deeper penetration of active ingredients. The other one is micro-needle mesotherapy which is nonsurgical therapy, which could cause the controlled inflammation. Micro channels are formed by needles during the skin puncture, that facilitate penetration of the active ingredients.

Aims: The aim of the study was to assess the efficacy of L-ascorbic acid applied in combination with no-needle and micro-needle mesotherapy in anti-aging therapy.

Materials and methods: This study involved 17 healthy volunteers, 2.5 ml of serum containing 20% L-ascorbic acid with hydrate from strawberries was used topically in every of 4 treatments. No-needle mesotherapy was applied on the left half of the face while microneedle mesotherapy in combination with the same serum was performed on the right half of face.

Results: In vivo studies confirmed the effectiveness of both methods.

Conclusion: The impact of active substance on skin firmness and elasticity as well as the degree of hydration and skin tone was more efficient after micro-needle mesotherapy.

Keywords: l-ascorbic acid; mesotherapy; preliminary trial; skin barrier; skin elasticity; skin lightening.

Publication types

  • Randomized Controlled Trial

MeSH terms

  • Aged
  • Ascorbic Acid / administration & dosage*
  • Combined Modality Therapy
  • Cosmetic Techniques*
  • Elasticity
  • Epidermis / drug effects*
  • Face
  • Female
  • Healthy Volunteers
  • Humans
  • Mesotherapy / instrumentation
  • Mesotherapy / methods*
  • Middle Aged
  • Needles
  • Rejuvenation
  • Skin Aging / drug effects*
  • Treatment Outcome


  • Ascorbic Acid