Hairy roots were induced in Althea officinalis using Agrobacterium rhizogenes, strains A4, A13, ATCC15834, and ATCC15834(GUS). The leaf, petiole and shoot explants of marshmallow were used for the hairy roots induction. When hairy roots appeared, cultures were established in MS (Murashige and Skoog) liquid medium without growth regulators. Hairy roots in explants appeared 5-12 days after inoculation. Maximum transformation frequency of 83% was observed on shoot explants with ATCC15834 strain. Among the strains, ATCC15834(GUS) strain showed better potential in the mass production of hairy roots in the hormone-free liquid medium after 50 days of culturing. The highest total phenolic and flavonoids content was found at 1.57 ± 0.1 mg/g dry weight in A13 strain and 3.47 ± 0.3 mg/g in A4 strain, respectively. Secondary metabolite content of hairy roots was found to be strain-specific.
Keywords: Agrobacterium rhizogenes; Althaea officinalis; Secondary metabolites; Transgenic roots; rolB.