Towards better understanding of industrial cell factories: novel approaches for 13C metabolic flux analysis in complex nutrient environments

Curr Opin Biotechnol. 2018 Dec:54:128-137. doi: 10.1016/j.copbio.2018.07.001. Epub 2018 Aug 2.


The quantification of intracellular fluxes via 13C metabolic flux analysis has become one of the major techniques to support systems-based metabolic engineering of industrial production hosts. Commonly, 13C studies are conducted using minimal media, however, a great variety of industrial processes rely on complex medium compounds. The use of parallel 13C tracer experiments as well as analytical multi-readout of labeling data together with model-based simulation offer a great starting point to tackle such complex systems. Prominent examples will highlight the recent progress made.

Publication types

  • Research Support, Non-U.S. Gov't
  • Review

MeSH terms

  • Antibody Formation
  • Biotechnology / methods*
  • Carbon Isotopes / metabolism*
  • Isotope Labeling / methods
  • Metabolic Engineering / methods
  • Metabolic Flux Analysis / methods*


  • Carbon Isotopes