Genetic and Biochemical Characterization of OXA-535, a Distantly Related OXA-48-Like β-Lactamase

L Dabos; A B Jousset; R A Bonnin; N Fortineau; A Zavala; P Retailleau; B I Iorga; T Naas

doi:10.1128/AAC.01198-18

Genetic and Biochemical Characterization of OXA-535, a Distantly Related OXA-48-Like β-Lactamase

Antimicrob Agents Chemother. 2018 Sep 24;62(10):e01198-18. doi: 10.1128/AAC.01198-18. Print 2018 Oct.

Authors

L Dabos^{1

2}, A B Jousset^{1

2

3

4}, R A Bonnin^{1

2

4}, N Fortineau^{1

2

3

4}, A Zavala⁵, P Retailleau⁵, B I Iorga⁵, T Naas^{6

2

3

4}

Affiliations

¹ EA7361 "Structure, Dynamic, Function and Expression of Broad Spectrum β-Lactamases," Paris-Sud University, Faculty of Medicine, Le Kremlin-Bicêtre, France.
² Joint Research Unit EERA Evolution and Ecology of Resistance to Antibiotics, Institut Pasteur-APHP-University Paris Sud, Paris, France.
³ Department of Bacteriology-Parasitology-Hygiene, Bicêtre Hospital, Assistance Publique-Hôpitaux de Paris, Le Kremlin-Bicêtre, France.
⁴ Associated French National Reference Center for Antibiotic Resistance, Le Kremlin-Bicêtre, France.
⁵ Institut de Chimie des Substances Naturelles, CNRS UPR 2301, Université Paris-Saclay, Labex LERMIT, Gif-sur-Yvette, France.
⁶ EA7361 "Structure, Dynamic, Function and Expression of Broad Spectrum β-Lactamases," Paris-Sud University, Faculty of Medicine, Le Kremlin-Bicêtre, France thierry.naas@aphp.fr.

Abstract

OXA-535 is a chromosome-encoded carbapenemase of Shewanella bicestrii JAB-1 that shares only 91.3% amino acid sequence identity with OXA-48. Catalytic efficiencies are similar to those of OXA-48 for most β-lactams, except for ertapenem, where a 2,000-fold-higher efficiency was observed with OXA-535. OXA-535 and OXA-436, a plasmid-encoded variant of OXA-535 differing by three amino acids, form a novel cluster of distantly related OXA-48-like carbapenemases. Comparison of bla_OXA-535 and bla_OXA-436 genetic environments suggests that an ISCR1 may be responsible for bla_OXA-436 gene mobilization from the chromosome of Shewanella spp. to plasmids.

Keywords: OXA-48; OXA-48-like; Shewanella; carbapenemase; progenitor.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Anti-Bacterial Agents / pharmacology
Bacterial Proteins / genetics
Bacterial Proteins / metabolism
Carbapenems / pharmacology
Chromosomes, Bacterial / genetics
Microbial Sensitivity Tests
Plasmids / genetics
Shewanella / drug effects
Shewanella / enzymology*
Shewanella / genetics
beta-Lactamases / genetics
beta-Lactamases / metabolism*
beta-Lactams / pharmacology

Substances

Anti-Bacterial Agents
Bacterial Proteins
Carbapenems
beta-Lactams
beta-Lactamases
carbapenemase