BODIPY-based fluorescent sensor for imaging of endogenous formaldehyde in living cells

Ting Cao; Deyan Gong; Shi-Chong Han; Anam Iqbal; Jing Qian; Wei Liu; Wenwu Qin; Huichen Guo

doi:10.1016/j.talanta.2018.07.001

BODIPY-based fluorescent sensor for imaging of endogenous formaldehyde in living cells

Talanta. 2018 Nov 1:189:274-280. doi: 10.1016/j.talanta.2018.07.001. Epub 2018 Jul 2.

Authors

Ting Cao¹, Deyan Gong¹, Shi-Chong Han², Anam Iqbal³, Jing Qian¹, Wei Liu¹, Wenwu Qin⁴, Huichen Guo⁵

Affiliations

¹ Key Laboratory of Nonferrous Metal Chemistry and Resources Utilization of Gansu Province and State Key Laboratory of Applied Organic Chemistry, College of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou 730000, PR China.
² State Key Laboratory of Veterinary Etiological Biology and Key Laboratory of Animal Virology of Ministry of Agriculture, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Xujiaping 1, Lanzhou, Gansu Province 730046, PR China.
³ Key Laboratory of Nonferrous Metal Chemistry and Resources Utilization of Gansu Province and State Key Laboratory of Applied Organic Chemistry, College of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou 730000, PR China; University of Balochistan, Quetta, Pakistan.
⁴ Key Laboratory of Nonferrous Metal Chemistry and Resources Utilization of Gansu Province and State Key Laboratory of Applied Organic Chemistry, College of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou 730000, PR China. Electronic address: qinww@lzu.edu.cn.
⁵ State Key Laboratory of Veterinary Etiological Biology and Key Laboratory of Animal Virology of Ministry of Agriculture, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Xujiaping 1, Lanzhou, Gansu Province 730046, PR China. Electronic address: guohuichen@caas.cn.

PMID: 30086918
DOI: 10.1016/j.talanta.2018.07.001

Abstract

A new highly selective fluorescent chemosensor for formaldehyde (FA) has been synthesized based on boron dipyrromethene as fluorophore and o-phenylenediamine (OPDA) as reaction group. When FA is added, the fluorescence emission band of the chemosensor red shift (from 525 nm to 548 nm) accompanied by an increase in intensity with strong green fluorescence was observed. This chemosensor also exhibited the lowest detection limit (0.104 μM) distinguished with other articles that have been reported. The application to cellular fluorescence imaging or test papers detection both indicated that the probe was highly responsive to the FA in endogenous cells and in the gaseous environment.

Keywords: BODIPY; Endogenous and exogenous; Fluorescent sensor; Formaldehyde; Gaseous detection.

MeSH terms

Boron Compounds / metabolism*
Cell Survival
Fluorescent Dyes / metabolism*
Formaldehyde / metabolism*
HeLa Cells
Humans
Limit of Detection
Optical Imaging / methods*

Substances

4,4-difluoro-4-bora-3a,4a-diaza-s-indacene
Boron Compounds
Fluorescent Dyes
Formaldehyde