Beta-adrenergic receptors on human suppressor, helper, and cytolytic lymphocytes

Biochem Pharmacol. 1986 Apr 1;35(7):1137-42. doi: 10.1016/0006-2952(86)90150-4.


Using the radioligand beta-adrenergic blocker [125I]cyanopindolol (ICYP), we have characterized the beta-adrenergic receptors on Leu 3+ (T helper [TH]), Leu 2+, 9.3- (T suppressor [Ts]) and Leu 2+, 9.3+ (T cytolytic [Tc]) subsets of human lymphocytes. Peripheral blood T cells were isolated by rosetting, and then subsets were purified by their affinities to monoclonal antibodies against their Leu 3 and 9.3 markers. ICYP binding to the subsets was saturable with time and with concentration; the binding was stereoselective and reversible by beta-adrenergic antagonists. A biological response produced by beta agonists increased intracellular concentrations of cAMP and corresponded to the number of binding sites. Each subset of cells had a number of binding sites, which was characteristic for the given subset. The data indicate that the density of distribution of beta-adrenergic receptors was not homogeneous on the precursors of phenotypically and functionally distinct T cells (Ts approximately 2900, Tc approximately 1800 and TH approximately 750 binding sites). The displacement studies using beta-adrenergic agonists were performed on the cytolytic and suppressor T cell subsets, suggesting that the receptors were mainly of the beta-2 type. The immunobiological significance of such selective distribution of numbers and subtypes of beta-adrenergic receptors on distinct T cell subsets is under investigation.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Epinephrine / metabolism
  • Humans
  • Iodocyanopindolol
  • Isoproterenol / metabolism
  • Norepinephrine / metabolism
  • Pindolol / analogs & derivatives
  • Pindolol / metabolism
  • Propranolol / metabolism
  • Receptors, Adrenergic, beta / metabolism*
  • Stereoisomerism
  • T-Lymphocytes, Cytotoxic / metabolism*
  • T-Lymphocytes, Helper-Inducer / metabolism*
  • T-Lymphocytes, Regulatory / metabolism*
  • Time Factors


  • Receptors, Adrenergic, beta
  • Iodocyanopindolol
  • Propranolol
  • Pindolol
  • Isoproterenol
  • Norepinephrine
  • Epinephrine