Effects of cannabidiol, a Cannabis sativa constituent, on oral wound healing process in rats: Clinical and histological evaluation

Phytother Res. 2018 Nov;32(11):2275-2281. doi: 10.1002/ptr.6165. Epub 2018 Aug 8.


The effects of cannabidiol (CBD), the main non-psychotropic component of Cannabis sativa, were assessed on oral wound healing in an in vivo model. Standardized ulcers were induced in 60 Wistar rats using a 5-mm biopsy punch on the midline of the ventral tongue. Animals received intraperitoneal injections of CBD at doses of 0 (control), 5, and 10 mg/kg daily. Animals were weighed daily, and wound healing was clinically and histologically evaluated after 3 and 7 days of treatment. CBD treatment did not influence the wound area of ulcerative lesions at either observation time. Conversely, microscopic findings revealed that at Day 3 postwounding, CBD-treated lesions exhibited significantly lower inflammatory scores than those in the control group. However, this difference was not observed at Day 7. Collectively, these findings indicate that CBD exert an antiinflammatory effect in early phase of wound healing process although it was not sufficient promote clinical improvement of oral traumatic ulcerative lesions.

Keywords: cannabidiol; cannabinoids; mouth diseases; oral ulcer; wound healing.

MeSH terms

  • Animals
  • Cannabidiol / pharmacology*
  • Cannabis / chemistry*
  • Injections, Intraperitoneal
  • Male
  • Oral Ulcer / drug therapy*
  • Rats
  • Rats, Wistar
  • Wound Healing / drug effects*


  • Cannabidiol