Nucleotide Sequence of Exon I of the Rat c-K-ras Gene

Bull Tokyo Med Dent Univ. 1986 Mar;33(1):35-40.

Abstract

We have isolated a DNA fragment containing exon I of rat cellular proto-oncogene of K-ras and sequenced the exon I. The obtained sequence was compared with that of the corresponding region in viral oncogene of Kirsten strain of murine sarcoma virus (Ki-MSV). The results showed that the exon I sequences of cellular K-ras genes in rat and human cells could encode the same amino acid sequence, while the viral K-ras gene could code for two different amino acids corresponding to the 12th and 37th positions from the N-terminus of K-ras gene product. The amino acid substitutions found between viral and cellular K-ras genes are discussed in relation to the transforming ability of Ki-MSV.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Base Sequence
  • Codon / genetics
  • DNA / genetics
  • DNA Restriction Enzymes
  • Humans
  • Nucleic Acid Hybridization
  • Oncogenes*
  • Proto-Oncogenes*
  • Rats
  • Rats, Inbred Strains

Substances

  • Codon
  • DNA
  • DNA Restriction Enzymes