Objective: To investigate effects of Biyuanshu( BYS) on molecular chaperone HSP70 and carboxyl terminus of HSC70 /HSP70-interacting protein( CHIP) expression of nasal sinuses mucosa epithele in mice Chronic rhinosinusitis( CRS) model, and to explore the BYS intervention mechanism from the point of molecular chaperone system.
Methods: 140 C57 male mice were randomly divided into normal group, sham operation group, model group, western medicine group, BYS low-dosage group, BYS medium-dosage group, BYS high-dosage group, with 20 mice in each group, and CRS model was established. With corresponding drug treatment for 14 days. Nasal sinuses mucosa tissue was collected to observe pathological alterations after HE dyeing, and HSP70 and its cofactor CHIP mRNA expression in nasal sinuses mucosa epithele were detected by real-time PCR, and the protein expression and IKK activity were detected by Western blotting.
Results: Model group appeared large necrotic and falling-off areas, apparently accompanied with chronic inflammatory cell infiltration. Nasal sinuses mucosa epithelial chaperon HSP70 and its cofactor CHIP expressions were much lower in CRS group than normal group and slam operation group( P < 0. 05 or P < 0. 01),p-IKKα / β expression in model group was obviously higher than normal group and slam operation group( P < 0. 01). Compared to model group, BYS medium-dosage and high-dosage groups presented well-repaired epithele in alignment, with fewer chronic inflammatory cell infiltration. Furthermore, expression of chaperon HSP70 and its cofactor CHIP in nasal sinuses mucosa epithelium were much higher than model group( P < 0. 01),but the p-IKKα / β expression was lower( P < 0. 01).
Conclusion: BYS can upregulate chaperon HSP70 and its cofactor CHIP to enhance intracellular protection from inflammatory protein injury mice, and reduce IKK activity to intervene on downstream NF-κB signaling pathway. BYS can be in favor of nasal sinuses mucosa epithelial repairmen.