Calcitonin-responsive clonal cell line from porcine kidney (PK(15)) in serum-free medium

Endocrinol Jpn. 1985 Dec;32(6):819-28. doi: 10.1507/endocrj1954.32.819.


PK(15), a homogeneous epithelial cell line from porcine kidney which was originally established through single cell cloning from PK-2a, was found to respond to [Asu1,7]eel calcitonin with an increase in adenosine 3':5'-cyclic monophosphate (cAMP) content, but do not respond to parathyroid hormone or arginine vasopressin. These cells were able to grow in a synthetic medium (a 1:1 mixture of Dulbecco's modified Eagle's MEM and Ham's F12 medium) without any supplementary factor. The medium supplemented with selenous acid, transferrin, and insulin permitted a growth rate equivalent to those in serum containing medium. When grown in the serum-free defined medium, these cells showed an increase in cAMP content in response to [Asu1,7]eel calcitonin to approximately the same degree as in the serum containing medium (10% fetal calf serum). Our present study first indicates that PK(15) cells are capable of growing in the serum-free defined medium retaining the calcitonin responsiveness of the original cells.

Publication types

  • Comparative Study

MeSH terms

  • Alkaline Phosphatase / metabolism
  • Animals
  • Arginine Vasopressin / pharmacology
  • Blood
  • Calcitonin / pharmacology*
  • Cell Division
  • Cell Line
  • Clone Cells
  • Culture Media
  • Cyclic AMP / metabolism*
  • Insulin / pharmacology
  • Kidney / cytology*
  • Kidney / enzymology
  • Kidney / metabolism
  • Male
  • Mice
  • Mice, Inbred C57BL
  • Parathyroid Hormone / pharmacology
  • Selenious Acid
  • Selenium / pharmacology
  • Swine
  • Transferrin / pharmacology


  • Culture Media
  • Insulin
  • Parathyroid Hormone
  • Transferrin
  • Arginine Vasopressin
  • Calcitonin
  • Cyclic AMP
  • Alkaline Phosphatase
  • Selenious Acid
  • Selenium