Effect of polymer charge on functional reconstitution of membrane proteins in polymer nanodiscs

Chem Commun (Camb). 2018 Aug 23;54(69):9615-9618. doi: 10.1039/c8cc04184a.


Although there is a growing interest in using polymer lipid-nanodiscs, the polymer charge poses limitations for studies on membrane proteins. Here, we demonstrate the functional reconstitution of a large soluble-domain containing positively-charged ∼57 kDa cytochrome-P450 and negatively-charged ∼16 kDa cytochrome-b5 in lipid-nanodiscs, and the role of the polymer charge for high-resolution studies on membrane proteins.