Radial glial elements in the cerebral cortex of the lesser hedgehog tenrec

Brain Struct Funct. 2018 Dec;223(9):3909-3917. doi: 10.1007/s00429-018-1730-1. Epub 2018 Aug 9.

Abstract

We investigated astroglial cells in several areas of the telencephalic cortex of the lesser hedgehog tenrec (Echinops telfairi). Compared to other mammals, the cortex of the tenrec has a relatively large paleocortex and a low encephalization index. We stained sections from tenrec forebrains with structural and functional glia markers focusing on selected cortical areas, the paleocortex, rhinal cortex, neocortex and the dentate gyrus of the hippocampal formation. We found that in all parts of the tenrec forebrain cortex, radial processes exist which are positive for glial fibrillary acidic protein (GFAP) although with differential localization: in the rhinal cortex and neocortical region radial glial fibers are located in the subventricular regions, whereas in the dentate gyrus and paleocortex they appear to arise from the cells in the respective granular layers. The relatively high abundance of the radial fibers in layer III of the paleocortex was very conspicuous. Only few of these radial processes were also co-labeled with doublecortin (DCX), yet most of the DCX-positive cells were negative for GFAP. The GFAP-positive radial fibers were in turn neither positive for glutamine synthetase, nor did they show immunoreactivity for the astroglia-specific water channel aquaporin-4 (AQP4). Star-shaped astrocytes, however, displayed the typical perivascular and subpial expression patterns for AQP4. We conclude that the radial glia in the adult tenrec represents an immature form of astroglia that persists in these animals throughout life.

Keywords: Afrotheria; Aquaporin-4; Astrocytes; Glia evolution.

MeSH terms

  • Animals
  • Aquaporin 4 / metabolism
  • Cerebral Cortex / cytology*
  • Cerebral Cortex / metabolism
  • Ependymoglial Cells / cytology*
  • Ependymoglial Cells / metabolism
  • Female
  • Glial Fibrillary Acidic Protein / metabolism
  • Insectivora / anatomy & histology*
  • Male
  • Microtubule-Associated Proteins / metabolism
  • Neuropeptides / metabolism

Substances

  • Aquaporin 4
  • Glial Fibrillary Acidic Protein
  • Microtubule-Associated Proteins
  • Neuropeptides
  • doublecortin protein