Wheat gluten hydrolysate potently stimulates peptide-YY secretion and suppresses food intake in rats

Biosci Biotechnol Biochem. 2018 Nov;82(11):1992-1999. doi: 10.1080/09168451.2018.1505482. Epub 2018 Aug 10.


The study was aimed to compare the satiating effect of various protein hydrolysates in rats and examine the underlying mechanism associated with the satiety hormones. Food intake and portal satiety hormone levels were measured in rats. Enteroendocrine cell-lines were employed to study the direct effect of protein hydrolysates on gut hormone secretions. The results showed that oral preload of wheat gluten hydrolysate (WGH) suppressed food intake greater and longer than other hydrolysates. The portal peptide-YY levels in WGH-treated rats at 2 h and 3 h were higher than those in control- and lactalbumin hydrolysate (LAH)-treated rats. In a distal enteroendocrine cell model, WGH more potently stimulated glucagon-like peptide-1 secretion than LAH, and the effect was largely enhanced by pepsin/pancreatin digestion of WGH. These results suggest WGH is potent in activating enteroendocrine cells to release satiety hormones leading to the prolonged suppression of food intake.

Keywords: Protein hydrolysate; enteroendocrine cell; food intake; gut satiety hormone.

MeSH terms

  • Animals
  • Cell Line
  • Enteroendocrine Cells / drug effects
  • Feeding Behavior / drug effects*
  • Gastrointestinal Hormones / blood
  • Gastrointestinal Hormones / metabolism
  • Glucagon-Like Peptide 1 / metabolism
  • Glutens / metabolism
  • Glutens / pharmacology*
  • Hydrolysis
  • Male
  • Mice
  • Pancreatin / metabolism
  • Pepsin A / metabolism
  • Peptide YY / metabolism*
  • Rats, Wistar
  • Satiation / drug effects
  • Triticum / chemistry*


  • Gastrointestinal Hormones
  • Peptide YY
  • Glutens
  • Pancreatin
  • Glucagon-Like Peptide 1
  • Pepsin A