Basolateral amygdala inactivation eliminates fear-induced underestimation of time in a temporal bisection task

Behav Brain Res. 2019 Jan 1;356:227-235. doi: 10.1016/j.bbr.2018.07.027. Epub 2018 Aug 8.

Abstract

We examined interval timing - time perception in the seconds-to-minutes range - of the fear-inducing stimulus and the role of the amygdala in this phenomenon. Rats were initially trained to perform a temporal bisection task, in which their responses to levers A and B were reinforced following 2-s and 8-s tones, respectively. After acquisition, the rats were also presented with tones of intermediate durations and pressed one of the two levers to indicate whether the tone duration was closer to 2 or 8 s. Subsequently, the rats underwent differential fear conditioning, in which one frequency tone (conditioned stimulus; CS+) was paired with an electric foot shock, whereas another frequency tone (CS-) was presented alone. The rats were then infused with artificial cerebrospinal fluid (aCSF) or the GABAA agonist muscimol into the bilateral basolateral amygdala (BLA) before performing the bisection task with CS+ and CS-. In rats infused with aCSF, the psychophysical function shifted rightward in CS+ relative to that in CS-. Moreover, the point of subjective equality of the CS+ was higher than that of CS-, suggesting that the duration of the fear -CS was perceived as shorter than that of the neutral CS. However, muscimol infusion into the BLA abolished this difference, suggesting that BLA inactivation suppresses the effect of the fear -CS. Our results demonstrate that normal BLA activity is essential for fear-induced underestimation of time.

Keywords: Amygdala; Fear; Interval timing; Temporal bisection task; Time perception.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amygdala / drug effects*
  • Animals
  • Basolateral Nuclear Complex / drug effects*
  • Conditioning, Classical / drug effects
  • Conditioning, Classical / physiology
  • Fear / physiology*
  • Freezing Reaction, Cataleptic / drug effects
  • GABA Agonists / pharmacology
  • Male
  • Muscimol / pharmacology
  • Rats, Wistar
  • Time Perception / drug effects

Substances

  • GABA Agonists
  • Muscimol