Generation of patient-specific induced pluripotent stem cell lines from one patient with Jervell and Lange-Nielsen syndrome, one with type 1 long QT syndrome and two healthy relatives

Stem Cell Res. 2018 Aug;31:174-180. doi: 10.1016/j.scr.2018.07.016. Epub 2018 Jul 25.


Four human iPSC cell lines (one Jervell and Lange-Nielsen Syndrome, one Long QT Syndrome-type 1 and two healthy controls) were generated from peripheral blood obtained from donors belonging to the same family. CytoTune™-iPS 2.0 Sendai Reprogramming Kit (containing OCT3/4, KLF4, SOX2 and cMYC as reprogramming factors) was used to generate all cell lines. The four iPSCs have normal karyotype, express pluripotency markers as determined by RT-PCR and flow cytometry and differentiated spontaneously in vitro into cells of the three germ layers, confirming their pluripotent capacity.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Differentiation
  • Humans
  • Induced Pluripotent Stem Cells / metabolism*
  • Jervell-Lange Nielsen Syndrome / genetics*
  • Jervell-Lange Nielsen Syndrome / pathology
  • Long QT Syndrome / complications*