Increasing branch and seed yield through heterologous expression of the novel rice S-acyl transferase gene OsPAT15 in Brassica napus L

Dan Peng; Xiaofeng Tan; Lin Zhang; Deyi Yuan; Jianzhong Lin; Xuanming Liu; Yueqiao Jiang; Bo Zhou

doi:10.1270/jsbbs.17126

Increasing branch and seed yield through heterologous expression of the novel rice S-acyl transferase gene OsPAT15 in Brassica napus L

Breed Sci. 2018 Jun;68(3):326-335. doi: 10.1270/jsbbs.17126. Epub 2018 Jul 5.

Authors

Dan Peng^{1

2}, Xiaofeng Tan^{3

4

5}, Lin Zhang^{3

4

5}, Deyi Yuan^{3

4

5}, Jianzhong Lin⁶, Xuanming Liu⁶, Yueqiao Jiang¹, Bo Zhou^{1

6

2}

Affiliations

¹ Faculty of Life Science and Technology, Central South University of Forestry and Technology, 410004, Changsha, China.
² Forestry Biotechnology Hunan Key Laboratories, 410004, Changsha, China.
³ Key Laboratory of Cultivation and Protection for Non-Wood Forest Trees, Ministry of Education, Central South University of Forestry and Technology, 410004, Changsha, China.
⁴ Key Laboratory of Cultivation and Protection for Non-Wood Forest Tree, Central South University of Forestry and Technology, 410004, Changsha, China.
⁵ Collaborative Innovation Central of Cultivation and Utilization for Non-Wood Forest Tree Central South University of Forestry and Technology, 410004, Changsha, China.
⁶ Key Laboratory of Plant Function Genomic for Development and Regulation, Hunan University, 410082, Changsha, China.

Abstract

Branching is a predominant element in the plant architecture of Brassica napus L. and represents an important determinant of seed yield. OsPAT15 (OsDHHC1), a novel DHHC-type zinc finger protein gene, was reported to regulate rice plant architecture by altering the tillering. However, whether heterologous overexpression of the OsPAT15 gene from the monocot rice into the dicot B. napus L. would have the same effect on branching or seed yield is unknown. In this study, the DHHC-type zinc finger protein gene OsPAT15 was determined to have sulfur acyl transferase activity in the akr1Δ yeast mutant in a complementation experiment. Heterologously expressing OsPAT15 transgenic B. napus L. plants were obtained using the Agrobacterium-mediated floral-dip transformation method. As anticipated, OsPAT15 transgenic plants exhibited branching and seed yield. Compared with non-transgenic plants, OsPAT15 transgenic plants had increased primary branches (1.58-1.76-fold) and siliques (1.86-1.89-fold), resulting in a significant increase in seed yield (around 2.39-2.51-fold). Therefore, overexpression of the sulfur acyl transferase gene OsPAT15 in B. napus L. could be used to increase seed yield and produce excellent varieties.

Keywords: B. napus L.; OsPAT15; branching; seed yield; sulfur acyl transferase.