B-1a Cell Development in Splenectomized Neonatal Mice

Front Immunol. 2018 Jul 30;9:1738. doi: 10.3389/fimmu.2018.01738. eCollection 2018.

Abstract

B-1a cells are mainly generated from fetal liver progenitor cells, peri- and neonatally. The developmental steps and anatomical sites required for these cells to become mature B-1a cells remain elusive. We recently described a phenotypically distinct transitional B cell subset in the spleen of neonatal mice that generated B-1a cells when adoptively transferred. This, in combination with findings demonstrating that B-1a cells are lacking in congenitally asplenic mice, led us to hypothesize that the neonatal spleen is required for B-1a cell development. In accordance with previous reports, we found that B-1a cell numbers were reduced in adult mice that had undergone splenectomy compared to after sham surgery. In contrast, neonatal splenectomy led to peritoneal B-1a cell frequencies comparable to those observed in sham-operated mice until 6 weeks after surgery, suggesting that an intact spleen is required for B-1a cell maintenance rather than development. To study the role of the prenatal spleen in generating B-1a cells, we transferred fetal liver cells from pre-splenic embryos [embryonic age 11 (E11) days] into splenectomized recipient mice. B-1a cells were generated in the absence of the spleen, albeit at slightly reduced frequencies, and populated the peritoneal cavity and bone marrow. Lower bone marrow B-1a cell frequencies were also observed both after neonatal and adult splenectomy. These results demonstrated that B-1a cells could be generated in the complete absence of an intact spleen, but that asplenia led to a decline in these cells, suggesting a role of the spleen for maintaining the B-1a compartment.

Keywords: B cell progenitors; B-1 cells; fetal liver; splenectomy; transitional B cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Age Factors
  • Animals
  • Animals, Newborn
  • B-Lymphocyte Subsets / cytology*
  • B-Lymphocyte Subsets / metabolism*
  • Biomarkers
  • Bone Marrow Cells / cytology
  • Bone Marrow Cells / metabolism
  • Cell Differentiation*
  • Cell Lineage
  • Immunophenotyping
  • Lymphocyte Count
  • Mice
  • Mice, Knockout
  • Mice, Transgenic
  • Phenotype
  • Precursor Cells, B-Lymphoid / cytology*
  • Precursor Cells, B-Lymphoid / metabolism*
  • Spleen / cytology
  • Spleen / immunology
  • Spleen / metabolism
  • Splenectomy*

Substances

  • Biomarkers