Effect of different mouthwashes on discoloration of plaque-free tooth surfaces

Am J Dent. 2018 Aug;31(4):211-214.


Purpose: To evaluate the tooth discoloration effect of the daily use protocol of six different mouthrinses.

Methods: 70 extracted caries-free central incisors were divided into seven groups (n=10/per group) : Group 1: Curasept Anti Discoloration System; Group 2: CB12; Group 3: Listerine Zero; Group 4: Listerine Cool Mint; Group 5: Colgate Plax; Group 6: Kloroben. The teeth were exposed to the mouthrinse for 2 minutes daily for 3 weeks. Color measurement was performed four times: at the beginning of the study, and after 1, 2 and 3 weeks, with a digital spectrophotometer (Vita Easyshade Advanced). Color changes (ΔE) were calculated and ANOVA and post hoc Tukey's test were used for statistical analysis.

Results: Although there was no statistically significant difference between the Colgate Plax and control groups, both groups had a significant difference from the other groups within all ΔE1, ΔE2 and ΔE3 values.

Clinical significance: Among the mouthrinses tested, Listerine Cool Mint group exhibited the most tooth discoloration, while Colgate Plax demonstrated the least noticeable color change.

MeSH terms

  • Dental Plaque
  • Humans
  • Mouthwashes* / adverse effects
  • Tooth
  • Tooth Bleaching Agents*
  • Tooth Discoloration* / chemically induced


  • Mouthwashes
  • Tooth Bleaching Agents