DELAYED HEADING DATE1 interacts with OsHAP5C/D, delays flowering time and enhances yield in rice

Plant Biotechnol J. 2019 Feb;17(2):531-539. doi: 10.1111/pbi.12996. Epub 2018 Sep 4.


Heading date is an important agronomic trait affecting crop yield. The GRAS protein family is a plant-specific super family extensively involved in plant growth and signal transduction. However, GRAS proteins are rarely reported have a role in regulating rice heading date. Here, we report a GRAS protein DHD1 (Delayed Heading Date1) delays heading and enhances yield in rice. Biochemical assays showed DHD1 physically interacts with OsHAP5C/D both in vitro and in vivo. DHD1 and OsHAP5C/D located in the nucleus and showed that rhythmic expression. Both DHD1 and OsHAP5C/D affect heading date by regulating expression of Ehd1. We propose that DHD1 interacts with OsHAP5C/D to delay heading date by inhibiting expression of Ehd1.

Keywords: Ehd1; GRAS; OsHAP5C/D; heading date; rice; yield.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Nucleus / metabolism
  • Down-Regulation
  • Flowers / genetics*
  • Flowers / growth & development
  • Flowers / physiology
  • Gene Expression Regulation, Plant / genetics*
  • Oryza / genetics*
  • Oryza / growth & development
  • Oryza / physiology
  • Phenotype
  • Plant Proteins / genetics
  • Plant Proteins / metabolism*
  • Plants, Genetically Modified
  • Quantitative Trait Loci / genetics*
  • Time Factors
  • Two-Hybrid System Techniques


  • Plant Proteins