The effects of bisphenol A on some plasma cytokine levels and distribution of CD8 + and CD4 + T lymphocytes in spleen, ileal Peyer's patch and bronchus associated lymphoid tissue in rats

Acta Histochem. 2018 Nov;120(8):728-733. doi: 10.1016/j.acthis.2018.08.002. Epub 2018 Aug 11.


The effects of bisphenol A on the some plasma cytokine levels and distribution of CD8+ and CD4+ T lymphocytes in spleen, ilealPeyer's patch and bronchus-associated lymphoid tissue in rats were investigated. A total of fourty male Wistar Albino rats were divided into five groups including 8 rats in each one: control, vehicle, BPA 5, BPA 50 and BPA 500 groups. Doses of 5, 50 and 500 μg/kg BPA were dissolved in ethanol, then mixed with corn oil. The control group received no treatment. The vehicle group was given the ethanol-corn oil mixture. BPA 5, BPA 50 and BPA 500 groups were given, respectively, 5, 50, and 500 μg/kg/day orally. In blood samples, IL-4, IL-6, IL-10 and TNF-α plasma levels were determined with ELISA. Tissue samples (spleen, ileal Peyer's patches and lung) were processed by means of routine histological techniques. CD4 and CD8 were stained immunohistochemically. Data obtained from this study showed that, BPA causes the alteration on immune parameters including cytokine profile, distribution of CD8+ and CD4+ T lymhpocytes in spleen and ileal Peyer's patches. Present study indicated that BPA may affect immune systems even at lower doses.Disruption of immun system cells and cytokine levels can result in harmful outcomes triggering autoimmune diseases and immunodeficiencies.

Keywords: Bisphenol A; Bronchus-associated lymphoid tissue; Cytokines; Ileal Peyer’s patches; Immunohistochemistry; Spleen.

MeSH terms

  • Administration, Oral
  • Animals
  • Benzhydryl Compounds / pharmacology*
  • Bronchi / chemistry*
  • Bronchi / immunology
  • CD4-Positive T-Lymphocytes / drug effects*
  • CD8-Positive T-Lymphocytes / drug effects*
  • Enzyme-Linked Immunosorbent Assay
  • Ileum / chemistry*
  • Ileum / immunology
  • Interleukin-4 / blood
  • Male
  • Peyer's Patches / drug effects*
  • Phenols / pharmacology*
  • Rats
  • Rats, Inbred WF
  • Reference Standards
  • Spleen / chemistry*
  • Spleen / immunology
  • Tumor Necrosis Factor-alpha / blood


  • Benzhydryl Compounds
  • Phenols
  • Tumor Necrosis Factor-alpha
  • Interleukin-4
  • bisphenol A