Cloning, characterization and nucleotide sequences of two cDNAs encoding human pancreatic trypsinogens

Gene. 1986;41(2-3):305-10. doi: 10.1016/0378-1119(86)90111-3.


Two cDNA clones encoding two major human trypsinogen isozymes were isolated from a human pancreatic cDNA library. The deduced amino acid (aa) sequences of the two trypsinogen precursors are found to have 89% sequence homology, and have the same number of aa (247), including 15 aa for a signal peptide and 8 aa for an activation peptide. Southern blot analysis of human genomic DNA using the cloned cDNA as a probe, revealed that the human trypsinogen genes constitute a multigene family of more than ten genes.

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • Cloning, Molecular*
  • DNA / isolation & purification*
  • DNA Restriction Enzymes
  • Humans
  • Isoenzymes / genetics*
  • Nucleic Acid Hybridization
  • Pancreas / enzymology*
  • Trypsinogen / genetics*


  • Isoenzymes
  • Trypsinogen
  • DNA
  • DNA Restriction Enzymes

Associated data

  • GENBANK/M22612