Cloning and characterization of the Schizosaccharomyces pombe DNA ligase gene CDC17

Gene. 1986;41(2-3):321-5. doi: 10.1016/0378-1119(86)90114-9.

Abstract

The Schizosaccharomyces pombe CDC17 gene has been cloned by complementation of the cdc17 mutant coding for temperature-sensitive DNA ligase. An allele-specific suppressor active only in the presence of a high osmotic pressure was also isolated. The cloned CDC17 gene failed to complement the analogous DNA ligase mutation, cdc9, in Saccharomyces cerevisiae, although the reverse complementation was successful [Barker and Johnston, Eur. J. Biochem. 134 (1983) 315-319]. The CDC17 gene specifies a 2.8-kb transcript.

MeSH terms

  • Cloning, Molecular*
  • DNA Ligases / genetics*
  • DNA Ligases / metabolism
  • DNA Restriction Enzymes
  • Genes*
  • Genes, Fungal*
  • Genetic Complementation Test
  • Mutation
  • Polynucleotide Ligases / genetics*
  • Saccharomycetales / genetics*
  • Schizosaccharomyces / enzymology
  • Schizosaccharomyces / genetics*
  • Temperature
  • Transcription, Genetic

Substances

  • DNA Restriction Enzymes
  • DNA Ligases
  • Polynucleotide Ligases