Wrapping-free numerical refocusing of scalar electromagnetic fields

Appl Opt. 2018 Aug 1;57(22):6582-6586. doi: 10.1364/AO.57.006582.

Abstract

Numerical refocusing in any plane is one powerful feature granted by measuring both the amplitude and the phase of a coherent light beam. Here, we introduce a method based on the first Rytov approximation of scalar electromagnetic fields that (i) allows numerical propagation without requiring phase unwrapping after propagation and (ii) limits the effect of artificial phase singularities that appear upon numerical defocusing when the measurement noise is mixing with the signal. We demonstrate the feasibility of this method with both scalar electromagnetic field simulations and real acquisitions of microscopic biological samples imaged at high numerical aperture.