Anti-androgenic therapy with finasteride improves cardiac function, attenuates remodeling and reverts pathologic gene-expression after myocardial infarction in mice

J Mol Cell Cardiol. 2018 Sep;122:114-124. doi: 10.1016/j.yjmcc.2018.08.011. Epub 2018 Aug 14.

Abstract

Maladaptive cardiac remodeling after myocardial infarction (MI) is increasingly contributing to the prevalence of chronic heart failure. Women show less severe remodeling, a reduced mortality and a better systolic function after MI compared to men. Although sex hormones are being made responsible for these differences, it remains currently unknown how this could be translated into therapeutic strategies. Because we had recently demonstrated that inhibition of the conversion of testosterone to its highly active metabolite dihydrotestosterone (DHT) by finasteride effectively reduces cardiac hypertrophy and improves heart function during pressure overload, we asked here whether this strategy could be applied to post-MI remodeling. We found increased abundance of DHT and increased expression of androgen responsive genes in the mouse myocardium after experimental MI. Treatment of mice with finasteride for 21 days (starting 7 days after surgery), reduced myocardial DHT levels and markedly attenuated cardiac dysfunction as well as hypertrophic remodeling after MI. Histological and molecular analyses showed reduced MI triggered interstitial fibrosis, reduced cardiomyocyte hypertrophy and increased capillary density in the myocardium of finasteride treated mice. Mechanistically, this was associated with decreased activation of myocardial growth-signaling pathways, a comprehensive normalization of pathological myocardial gene-expression as revealed by RNA deep-sequencing and with direct effects of finasteride on cardiac fibroblasts and endothelial cells. In conclusion, we demonstrated a beneficial role of anti-androgenic treatment with finasteride in post-MI remodeling of mice. As finasteride is already approved for the treatment of benign prostate disease, it could potentially be evaluated as therapeutic strategy for heart failure after MI.

Keywords: Cardiac hypertrophy; Myocardial remodeling; Signaling; Testosterone.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Analysis of Variance
  • Androgen Antagonists / therapeutic use*
  • Animals
  • Cardiomegaly / drug therapy
  • Cell Line
  • Dihydrotestosterone / metabolism
  • Endothelial Cells / drug effects
  • Fibroblasts / drug effects
  • Fibrosis
  • Finasteride / therapeutic use*
  • Gene Expression / drug effects*
  • Human Umbilical Vein Endothelial Cells
  • Humans
  • Induced Pluripotent Stem Cells
  • Male
  • Mice
  • Muscle Contraction / drug effects
  • Myocardial Infarction / drug therapy*
  • Myocardium / pathology
  • Neovascularization, Physiologic / drug effects
  • Rats
  • Rats, Sprague-Dawley
  • Ventricular Function, Left / drug effects*
  • Ventricular Remodeling / drug effects*

Substances

  • Androgen Antagonists
  • Dihydrotestosterone
  • Finasteride