Mapping of a second recombination hot spot within the I-E region of the mouse H-2 gene complex

J Exp Med. 1986 Jun 1;163(6):1518-28. doi: 10.1084/jem.163.6.1518.

Abstract

The crossover points of nine intra-I region recombinant mouse strains were determined by restriction fragment analysis. The recombinants were examined for the presence of k and p haplotype specific DNA restriction endonuclease sites. These restriction sites were a Sac I site between the E beta and E beta 2 genes, a Hpa I site within the E beta 2 gene, and a Rsa I site approximately 1 kb to the right of the E alpha gene. Seven of the recombinants were found to have crossovers between the Hpa I and the Rsa I site. This analysis suggests that a recombination hot spot exists within this segment. This segment is approximately 12-14 kb, and contains the E alpha gene and the intervening sequence between the E beta 2 and E alpha genes.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • DNA / analysis
  • DNA Restriction Enzymes
  • H-2 Antigens / genetics
  • Histocompatibility Antigens / genetics*
  • Histocompatibility Antigens Class II*
  • Major Histocompatibility Complex*
  • Mice
  • Oligodeoxyribonucleotides / analysis
  • Polymorphism, Genetic
  • Recombination, Genetic*

Substances

  • H-2 Antigens
  • Histocompatibility Antigens
  • Histocompatibility Antigens Class II
  • I-E-antigen
  • Oligodeoxyribonucleotides
  • DNA
  • DNA Restriction Enzymes