Evaluation of Regenerative Potential of Cryopreserved Adenosine-Modified Monocytes on the Model of Burn Wounds

K V Nevskaya; V V Ivanov; E S Mainagasheva; S S Gutor; S P Lezhava; A E Sazonov

doi:10.1007/s10517-018-4209-4

Evaluation of Regenerative Potential of Cryopreserved Adenosine-Modified Monocytes on the Model of Burn Wounds

Bull Exp Biol Med. 2018 Aug;165(4):526-529. doi: 10.1007/s10517-018-4209-4. Epub 2018 Aug 18.

Authors

K V Nevskaya¹, V V Ivanov², E S Mainagasheva², S S Gutor², S P Lezhava², A E Sazonov^{2

3}

Affiliations

¹ Siberian State Medical University, Ministry of Health of the Russian Federation, Tomsk, Russia. nevskayaksenia@gmail.com.
² Siberian State Medical University, Ministry of Health of the Russian Federation, Tomsk, Russia.
³ D. I. Mendeleev University of Chemical Technology, Moscow, Russia.

PMID: 30121925
DOI: 10.1007/s10517-018-4209-4

Abstract

We studied viability of adenosine-modified monocytes after cryopreservation and their regenerative potential. The optimal method of cell cryopreservation was the use of serum-free medium followed by storage in liquid nitrogen. Injections of adenosine-modified monocytes after 12-week cryopreservation around grade IIIB burn wound accelerated healing of the skin defect and significantly improved the histological pattern of the damage area at delayed terms.

Keywords: adenosine; burn; cryopreservation; monocytes; regeneration.

MeSH terms

Adenosine / pharmacology*
Adult
Animals
Burns / metabolism
Cryopreservation / methods*
Humans
Monocytes / cytology*
Monocytes / drug effects
Monocytes / physiology*
Rats
Skin Diseases / metabolism

Substances

Adenosine