Cell-Surface Marker Signature for Enrichment of Ventricular Cardiomyocytes Derived from Human Embryonic Stem Cells

Stem Cell Reports. 2018 Sep 11;11(3):828-841. doi: 10.1016/j.stemcr.2018.07.007. Epub 2018 Aug 16.


To facilitate understanding of human cardiomyocyte (CM) subtype specification, and the study of ventricular CM biology in particular, we developed a broadly applicable strategy for enrichment of ventricular cardiomyocytes (VCMs) derived from human embryonic stem cells (hESCs). A bacterial artificial chromosome transgenic H9 hESC line in which GFP expression was driven by the human ventricular-specific myosin light chain 2 (MYL2) promoter was generated, and screened to identify cell-surface markers specific for MYL2-GFP-expressing VCMs. A CD77+/CD200- cell-surface signature facilitated isolation of >97% cardiac troponin I-positive cells from H9 hESC differentiation cultures, with 65% expressing MYL2-GFP. This study provides a tool for VCM enrichment when using some, but not all, human pluripotent stem cell lines. Tools generated in this study can be utilized toward understanding CM subtype specification, and enriching for VCMs for therapeutic applications.

Keywords: cardiac differentiation; cell-surface marker signature; human embryonic stem cells; ventricular cardiomyocytes.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antigens, CD / analysis
  • Cardiac Myosins / analysis
  • Cell Differentiation
  • Cell Line
  • Cells, Cultured
  • Heart Ventricles / cytology*
  • Human Embryonic Stem Cells / cytology*
  • Humans
  • Myocytes, Cardiac / cytology*
  • Myosin Light Chains / analysis
  • Trihexosylceramides / analysis


  • Antigens, CD
  • Myosin Light Chains
  • Trihexosylceramides
  • myosin light chain 2
  • globotriaosylceramide
  • Cardiac Myosins
  • antigens, CD200