Elevated arterial lactate delays recovery of intracellular muscle pH after exercise

Abstract

Purpose: We evaluated muscle proton elimination following similar exercise in the same muscle group following two exercise modalities.

Methods: Seven rowers performed handgrip or rowing exercise for ~ 5 min. The intracellular response of the wrist flexor muscles was evaluated by ³¹P nuclear magnetic resonance spectroscopy, while arterial and venous forearm blood was collected.

Results: Rowing and handgrip reduced intracellular pH to 6.3 ± 0.2 and 6.5 ± 0.1, arterial pH to 7.09 ± 0.03 and 7.40 ± 0.03 and venous pH to 6.95 ± 0.06 and 7.20 ± 0.04 (P < 0.05), respectively. Arterial and venous lactate increased to 17.5 ± 1.6 and 20.0 ± 1.6 mM after rowing while only to 2.6 ± 0.8 and 6.8 ± 0.8 mM after handgrip exercise. Arterio-venous concentration difference of bicarbonate and phosphocreatine recovery kinetics (T_50% rowing 1.5 ± 0.7 min; handgrip 1.4 ± 1.0 min) was similar following the two exercise modalities. Yet, intramuscular pH recovery in the forearm flexor muscles was 3.5-fold slower after rowing than after handgrip exercise (T_50% rowing of 2 ± 0.1 vs. 7 ± 0.3 min for handgrip).

Conclusion: Rowing delays intracellular-pH recovery compared with handgrip exercise most likely because rowing, as opposed to handgrip exercise, increases systemic lactate concentration. Thus the intra-to-extra-cellular lactate gradient is small after rowing. Since this lactate gradient is the main driving force for intracellular lactate removal in muscle and, since pH_i normalization is closely related to intracellular lactate removal, rowing results in a slower pH_i recovery compared to handgrip exercise.

Keywords: 31P-magnetic resonance spectroscopy (31PMRS); Handgrip; Muscle pH; Rowing.