Automated dendritic spine detection using convolutional neural networks on maximum intensity projected microscopic volumes

J Neurosci Methods. 2018 Nov 1;309:25-34. doi: 10.1016/j.jneumeth.2018.08.019. Epub 2018 Aug 18.

Abstract

Background: Dendritic spines are structural correlates of excitatory synapses in the brain. Their density and structure are shaped by experience, pointing to their role in memory encoding. Dendritic spine imaging, followed by manual analysis, is a primary way to study spines. However, an approach that analyses dendritic spines images in an automated and unbiased manner is needed to fully capture how spines change with normal experience, as well as in disease.

New method: We propose an approach based on fully convolutional neural networks (FCNs) to detect dendritic spines in two-dimensional maximum-intensity projected images from confocal fluorescent micrographs. We experiment on both fractionally strided convolution and efficient sub-pixel convolutions. Dendritic spines far from the dendritic shaft are pruned by extraction of the shaft to reduce false positives. Performance of the proposed method is evaluated by comparing predicted spine positions to those manually marked by experts.

Results: The averaged distance between predicted and manually annotated spines is 2.81 ± 2.63 pixels (0.082 ± 0.076 microns) and 2.87 ± 2.33 pixels (0.084 ± 0.068 microns) based on two different experts. FCN-based detection achieves F scores > 0.80 for both sets of expert annotations.

Comparison with existing methods: Our method significantly outperforms two well-known software, NeuronStudio and Neurolucida (p-value < 0.02).

Conclusions: FCN architectures used in this work allow for automated dendritic spine detection. Superior outcomes are possible even with small training data-sets. The proposed method may generalize to other datasets on larger scales.

Keywords: Convolutional neural networks; Deep learning; Dendritic spine detection.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Dendritic Spines*
  • Imaging, Three-Dimensional / methods
  • Male
  • Mice
  • Microscopy, Confocal / methods*
  • Neural Networks, Computer*
  • Pattern Recognition, Automated / methods*