Isolation of Two Lapine Rotaviruses: Characterization of Their Subgroup, Serotype and RNA Electropherotypes

Arch Virol. 1986;89(1-4):161-70. doi: 10.1007/BF01309886.

Abstract

Rotaviruses were detected by an ELISA test in stool specimens from diarrheic rabbits in two commercial rabbitries and cultured in MA 104 cells. Their identity was confirmed by electron microscopy and indirect immunofluorescence. They were found to belong to subgroup I by testing with monoclonal antibodies and to serotype 3 by neutralization with homologous and heterologous antisera. Although both viruses were neutralized by antiserum to human serotype 3 the ALA rabbit rotavirus was minimally neutralized by antiserum to the C11 rabbit rotavirus. Electrophoresis of viral RNA revealed 11 segments characteristic of rotavirus, however both rabbit rotaviruses had unusual electropherotypes. They differed from each other with greatly reduced mobility of the tenth segment in one virus and the eleventh segment in the other virus.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Cell Line
  • Culture Techniques / methods
  • Electrophoresis, Polyacrylamide Gel
  • Enzyme-Linked Immunosorbent Assay
  • Fluorescent Antibody Technique
  • Immune Sera
  • Neutralization Tests
  • RNA, Viral / isolation & purification*
  • Rabbits
  • Rotavirus / classification
  • Rotavirus / isolation & purification*
  • Serotyping

Substances

  • Immune Sera
  • RNA, Viral