Hyperacute serum has markedly better regenerative efficacy than platelet-rich plasma in a human bone oxygen-glucose deprivation model

Regen Med. 2018 Jul 1;13(5):531-543. doi: 10.2217/rme-2017-0141. Epub 2018 Aug 22.


Aim: Platelet-rich plasma (PRP) and hyperacute serum (HAS) were compared in a novel human model of ex vivo bone damage induced by oxygen-glucose deprivation (OGD).

Materials & methods: Osteoarthritic subchondral bone pieces were harvested from discarded femoral heads during hip replacement surgery and subjected to transient OGD.

Results: Proteome profiling revealed that PRP is more angiopoietic, whereas HAS is more antiangiopoietic in composition. However, treatment of OGD-exposed bone with multiple PRP preparations had no effect on cell counts, whereas HAS restored cell proliferation capacity and rescued viable cell number following OGD.

Conclusion: A similar pro-proliferation effect was observed with recombinant growth factors, indicating that HAS may be an alternative agent for enhancing the regeneration of damaged bone cells.

Keywords: blood fractions; bone necrosis; femoral head; growth factors; ischemia.

MeSH terms

  • Femur Head / cytology
  • Femur Head / metabolism*
  • Glucose / metabolism
  • Humans
  • Organ Culture Techniques
  • Oxygen / metabolism
  • Platelet-Rich Plasma*
  • Proteome / metabolism*
  • Serum*


  • Proteome
  • Glucose
  • Oxygen