Experimental chronic heart failure (CHF), caused by administration of L-isoproterenol (2.5 mg/kg twice a day intraperitoneally for 21 days), promotes uncoupling of respiration and oxidative phosphorylation. The rate of mitochondrial oxygen consumption in the metabolic state V3 by Chance in animals with CHF decreased by 53.3% (p<0.05) with malate using (as an oxidation substrate feeding сomplex I of the electron transport chain (ETC)), by 70.6% (p<0.05) with succinate using (сomplex II substrate) and by 63.6% (p<0.05) when malate and succinate were added simultaneously. The respiratory control ratio significantly decreased 2.3 times for сomplex I, 2.5 for сomplex II, and 2.6 times for the simultaneous operation of two respiratory chain complexes in mitochondria of CHF rats compared to intact animals. Mitochondrial dysfunction in experimental CHF is evidently due to the development of oxidative stress. It was revealed that the content of malonic dialdehyde (MDA) in the group of rats with experimental CHF was higher by 54.7% (p<0.05), as compared with intact animals. The activity of superoxide dismutase (SOD) and catalase was lower by 17.5% (p<0.05), and by 18.4%, respectively than in the intact group. The dense extract from herba of Primula veris L. (DEHPV) 30 mg/kg limits the development of mitochondrial dysfunction in rats with experimental CHF, as evidenced by an increase in the role of V3 respiration for the first and second respiratory chain complexes in 1.7 (p<0.05) and 2.0 times (p<0.05), respectively, the ratio of respiratory control (RCR) - 1.7 times (p<0.05) for сomplex I and 2 times (p<0.05) for сomplex II compared with the negative control. The concentration of MDA was by 15.7% (p<0.05), lower and the activity of SOD was by 56.3% (p<0.05) higher.
Pokazano, chto éksperimental'naia khronicheskaia serdechnaia nedostatochnost' (KhSN), vyzvannaia vvedeniem L-izoproterenola v doze 2,5 mg/kg dvazhdy v sutki vnutribriushinno v techenie 21 dnia, sposobstvuet razobshcheniiu dykhaniia i okislitel'nogo fosforilirovaniia v mitokhondriiakh kletok serdtsa, na chto ukazyvaet snizhenie skorosti potrebleniia kisloroda v metabolicheskom sostoianii V3 po Chansu u zhivotnykh s KhSN na 53,3% (p<0,05) pri ispol'zovanii malata (substrata okisleniia kompleksa I tsepi perenosa élektronov (TsPÉ)), na 70,6% (p<0,05) – suktsinata (substrata kompleksa II TsPÉ) i na 63,6% (p<0,05) pri ikh odnovremennom primenenii. Koéffitsient dykhatel'nogo kontrolia (KDK) dostoverno snizhalsia v 2,3 raza dlia kompleksa I, v 2,5 – dlia kompleksa II i v 2,6 raza pri odnovremennoĭ rabote dvukh kompleksov dykhatel'noĭ tsepi u krys s éksperimental'noĭ KhSN po sravneniiu s intaktnymi zhivotnymi. Mitokhondrial'naia disfunktsiia pri éksperimental'noĭ KhSN, ochevidno, obuslovlena razvitiem oksidativnogo stressa. Vyiavleno, chto soderzhanie malonovogo dial'degida (MDA) v gruppe krys s éksperimental'noĭ KhSN, bylo vyshe na 54,7% (p<0,05) po sravneniiu s pokazateliami intaktnykh zhivotnykh. Pri étom summarnaia aktivnost' superoksiddismutazy (SOD) v kontrol'noĭ gruppe byla na 17,5% (p<0,05), katalazy – na 18,4% nizhe po sravneniiu s intaktnoĭ gruppoĭ. Gustoĭ ékstrakt iz travy pervotsveta vesennego (GÉTPV) ogranichivaet razvitie mitokhondrial'noĭ disfunktsii u krys s éksperimental'noĭ KhSN, o chem svidetel'stvuet povyshenie V3 dlia pervogo i vtorogo kompleksov dykhatel'noĭ tsepi na 77,2 (p<0,05) i 114,6% (p<0,05) sootvetstvenno, KDK – v 1,7 raza (p<0,05) dlia kompleksa I i v 2 raza (p<0,05) dlia kompleksa II po sravneniiu s negativnym kontrolem. Pri étom kontsentratsiia MDA byla na 15,7% (p<0,05) nizhe, a summarnaia aktivnost' SOD na 56,3% (p<0,05) vyshe.
Keywords: chronic heart failure; dense extract from herba of Primula veris; mitochondrial dysfunction; oxidative stress.