Exploring PEGylated and immobilized laccases for catechol polymerization

AMB Express. 2018 Aug 22;8(1):134. doi: 10.1186/s13568-018-0665-5.


Laccases have been reported for their ability to eliminate hazardous phenolic compounds by oxidative polymerization. The exploitation of the oxidative behavior of different laccase forms, namely free/native, free/PEGylated, immobilized/native and immobilized/PEGylated, was assessed in this study. We found that PEGylated and immobilized laccase forms have differentiated catalytic behavior revealing distinct conversion rates and differentiated poly(catechol) chains, as confirmed by UV-Visible spectroscopy, by the total content of OH groups and by MALDI-TOF spectroscopy. The synergy underlying on the immobilized/PEGylated enzyme forms reveal to be responsible for the highest conversion rates and for the longer polymers produced.

Keywords: Immobilization; Laccase; PEGylation; Polyethylene glycol; Polymerization.