Molecular cloning of cDNA for rat liver gap junction protein

J Cell Biol. 1986 Jul;103(1):123-34. doi: 10.1083/jcb.103.1.123.


An affinity-purified antibody directed against the 27-kD protein associated with isolated rat liver gap junctions was produced. Light and electron microscopic immunocytochemistry showed that this antigen was localized specifically to the cytoplasmic surfaces of gap junctions. The antibody was used to select cDNA from a rat liver library in the expression vector lambda gt11. The largest cDNA selected contained 1,494 bp and coded for a protein with a calculated molecular mass of 32,007 daltons. Northern blot analysis indicated that brain, kidney, and stomach express an mRNA with similar size and homology to that expressed in liver, but that heart and lens express differently sized, less homologous mRNA.

Publication types

  • Comparative Study
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Cloning, Molecular
  • Connexins
  • DNA / genetics
  • Immunologic Techniques
  • Intercellular Junctions*
  • Liver / physiology
  • Membrane Proteins / genetics*
  • Membrane Proteins / immunology
  • Nucleic Acid Hybridization
  • Protein Conformation
  • Rats
  • Recombinant Proteins / genetics
  • Recombinant Proteins / immunology
  • Tissue Distribution


  • Connexins
  • Membrane Proteins
  • Recombinant Proteins
  • DNA

Associated data

  • GENBANK/X04070