Downregulation of hsa_circ_0007534 suppresses breast cancer cell proliferation and invasion by targeting miR-593/MUC19 signal pathway

Biochem Biophys Res Commun. 2018 Sep 18;503(4):2603-2610. doi: 10.1016/j.bbrc.2018.08.007. Epub 2018 Aug 20.

Abstract

Circular RNAs (circRNAs) are important non-coding RNAs that are reportedly involved in the progression of diverse human cancers through their action as a microRNA (miRNA) sponge. However, the exact roles of circRNAs in breast cancer (BC) remain largely unknown. The present data demonstrate the significantly upregulated expression of hsa_circ_0007534 circRNA in BC tissues and cell lines. In contrast, miR-593 expression was significantly downregulated. Knockdown of hsa_circ_0007534 inhibited BC cell proliferation, colony formation, and invasion, and promoted apoptosis in BC cells. Moreover, hsa_circ_0007534 was demonstrated to be a sponge of miR-593, and expression of miR-593 in BC cells was negatively correlated with hsa_circ_0007534. MUC19 expression was markedly increased in BC tissues and cell lines, and the 3'-UTR of MUC19 was targeted by miR-593. The expression of MUC19 was negatively regulated by miR-593 in BC cells. Our findings suggest an oncogenic role for hsa_circ_0007534 in BC by acting as a miR-593 sponge to promote MUC19 expression.

Keywords: Apoptosis; Breast cancer (BC); MUC19; Proliferation; hsa_circ_0007534; miR-593.

MeSH terms

  • 3' Untranslated Regions
  • Apoptosis
  • Base Sequence
  • Breast Neoplasms / genetics*
  • Breast Neoplasms / metabolism
  • Breast Neoplasms / mortality
  • Breast Neoplasms / pathology
  • Cell Cycle
  • Cell Line, Tumor
  • Cell Movement
  • Cell Proliferation
  • Epithelial Cells / metabolism
  • Epithelial Cells / pathology
  • Female
  • Gene Expression Regulation, Neoplastic*
  • Humans
  • MicroRNAs / genetics*
  • MicroRNAs / metabolism
  • Mucins / genetics*
  • Mucins / metabolism
  • Oligoribonucleotides, Antisense / genetics
  • Oligoribonucleotides, Antisense / metabolism
  • RNA / genetics*
  • RNA / metabolism
  • RNA, Circular
  • RNA, Neoplasm / genetics*
  • RNA, Neoplasm / metabolism
  • Signal Transduction
  • Survival Analysis

Substances

  • 3' Untranslated Regions
  • MIRN593 microRNA, human
  • MUC19 protein, human
  • MicroRNAs
  • Mucins
  • Oligoribonucleotides, Antisense
  • RNA, Circular
  • RNA, Neoplasm
  • RNA