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. 2019 May;140(2):129-139.
doi: 10.1007/s11120-018-0572-2. Epub 2018 Aug 23.

Circular Spectropolarimetric Sensing of Higher Plant and Algal Chloroplast Structural Variations

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Free PMC article

Circular Spectropolarimetric Sensing of Higher Plant and Algal Chloroplast Structural Variations

C H Lucas Patty et al. Photosynth Res. .
Free PMC article

Abstract

Photosynthetic eukaryotes show a remarkable variability in photosynthesis, including large differences in light-harvesting proteins and pigment composition. In vivo circular spectropolarimetry enables us to probe the molecular architecture of photosynthesis in a non-invasive and non-destructive way and, as such, can offer a wealth of physiological and structural information. In the present study, we have measured the circular polarizance of several multicellular green, red, and brown algae and higher plants, which show large variations in circular spectropolarimetric signals with differences in both spectral shape and magnitude. Many of the algae display spectral characteristics not previously reported, indicating a larger variation in molecular organization than previously assumed. As the strengths of these signals vary by three orders of magnitude, these results also have important implications in terms of detectability for the use of circular polarization as a signature of life.

Keywords: Algae; Chlorophyll; Chloroplast; Circular polarization; Photosynthesis.

Figures

Fig. 1
Fig. 1
Evolutionary relationships based on the host rRNA (left) and based on chloroplast DNA (cpDNA) (right)
Fig. 2
Fig. 2
Schematic representation of the photosynthetic structures of higher plants and algae. There is a distinct organizational difference in the supercomplexes between higher plants and algae. Additionally, while green algae display stacked thylakoid membranes, they lack true grana. Red algae contain phycobilisomes, unlike the other algae. In brown algae the thylakoid membranes are threefold and the supercomplex organization is not entirely resolved
Fig. 3
Fig. 3
Circular polarimetric spectra of S. japonica, P. laurocerasus, and A. elatior leaves. Shaded areas denote the standard error, n = 3 per species
Fig. 4
Fig. 4
Circular polarimetric spectra of U. lactuca and Ulva sp. green algae. Shaded areas denote the standard error, n = 3 per species
Fig. 5
Fig. 5
Circular polarimetric spectra of Porphyra sp. and G. turuturu red algae. Shaded areas denote the standard error, n = 3 per species
Fig. 6
Fig. 6
Circular polarimetric spectra of S. latissima (juvenile and mature) and U. pinnatifida brown algae. Shaded areas denote the standard error, n = 3 per species
Fig. 7
Fig. 7
Circular polarimetric spectra of F. serratus and F. spiralis brown algae. Shaded areas denote the standard error, n = 3 per species
Fig. 8
Fig. 8
Maximum extend of the V/I bands within 650 nm to 700 nm against the absorbance over 675 nm to 685 nm. Error bars denote the standard error for n = 3 per species
Fig. 9
Fig. 9
The three major sources of circular polarizance around the chlorophyll absorbance band in the red for higher plants for identical chlorophyll concentrations. Adapted after (Garab and van Amerongen 2009)

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