Investigation of Nuclear Periphery Protein Interactions in Plants Using the Membrane Yeast Two-Hybrid (MbY2H) System

Methods Mol Biol. 2018:1840:221-235. doi: 10.1007/978-1-4939-8691-0_16.

Abstract

Identification of membrane protein interactomes is a key issue to better understand how these molecules carry out their functions. However, protein-protein interactions using conventional interaction assays are particularly challenging for integral membrane proteins, because of their hydrophobic nature. Here we describe the membrane yeast two-hybrid (MbY2H) system, a powerful tool for identifying the interactors of membrane and membrane-associated proteins.

Keywords: Arabidopsis thaliana; Interactome; LINC complex; Membrane protein; Yeast two-hybrid.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Nucleus / metabolism*
  • Cloning, Molecular
  • Gene Expression
  • Gene Library
  • Genetic Vectors
  • Membrane Proteins / metabolism*
  • Nuclear Proteins / metabolism*
  • Plant Cells / metabolism*
  • Protein Binding
  • Protein Interaction Mapping / methods*
  • Two-Hybrid System Techniques*

Substances

  • Membrane Proteins
  • Nuclear Proteins