Vascular vasopressin receptors mediate phosphatidylinositol turnover and calcium efflux in an established smooth muscle cell line

Life Sci. 1986 Jul 7;39(1):37-45. doi: 10.1016/0024-3205(86)90435-2.


Vasopressin-induced phosphatidylinositol turnover and mobilization of intracellular Ca2+ was studied using an established smooth muscle cell line (A-10). The cells were subcloned to ensure a monoclonal cell population. The accumulation of inositol mono-, di-, and tris-phosphates (IP1, IP2, and IP3, respectively), and the mobilization of intracellular Ca2+ were dependent on the time of incubation and the concentration of arginine vasopressin (AVP). IP1, IP2, and IP3 were significantly elevated after 15 sec and remained elevated for up to 2 hr. The concentrations of AVP required for half-maximal stimulation of IP1, IP2, and IP3 formation were 2, 12, and 4 nM, respectively. LiCl was required to observe the accumulation of inositol phosphates in response to AVP. Significant 45Ca2+ efflux was observed within 15 sec after exposure to AVP. By employing the vasopressin receptor subtype selective antagonists [d(CH2)5Tyr(Me)AVP, V1; d(CH2)5D-Tyr(Et)VAVP,V1/V2; d(CH2) 5D-IleVAVP,V2] and agonists [AVP, V1/V2; dDAVP, V2; dVDAVP, V2], we found that the vasopressin-induced stimulation of phosphatidylinositol turnover and 45Ca2+ efflux were mediated by receptors of the vascular V1 subtype. Pertussis toxin pretreatment partially inhibited vasopressin-induced phosphatidylinositol turnover. These data demonstrate that activation of V1 receptors of vascular smooth muscle cells resulted in enhanced phosphatidylinositol turnover and mobilization of intracellular Ca2+.

MeSH terms

  • Angiotensin II / pharmacology
  • Animals
  • Aorta, Thoracic
  • Arginine Vasopressin / pharmacology
  • Calcium / metabolism*
  • Cell Line
  • Muscle, Smooth, Vascular / drug effects*
  • Muscle, Smooth, Vascular / metabolism
  • Pertussis Toxin
  • Phenylephrine / pharmacology
  • Phosphatidylinositols / metabolism*
  • Rats
  • Receptors, Angiotensin / drug effects
  • Receptors, Angiotensin / physiology*
  • Receptors, Cell Surface / physiology*
  • Receptors, Vasopressin
  • Vasopressins / pharmacology*
  • Virulence Factors, Bordetella / pharmacology


  • Phosphatidylinositols
  • Receptors, Angiotensin
  • Receptors, Cell Surface
  • Receptors, Vasopressin
  • Virulence Factors, Bordetella
  • Vasopressins
  • Angiotensin II
  • Arginine Vasopressin
  • Phenylephrine
  • Pertussis Toxin
  • Calcium